Graduation of Xiaonai Senior Sister Apprentice's Master 小娜师姐硕士毕业

PCGF6 Regulates Stem Cell Pluripotency via Super-Enhancer Dependent Chromatin Interactions        Polycomb group (PcG) ring finger protein 6 (PCGF6), known as a core component of the transcription-repressing complexes, PcG, canonically acts as a transcriptional repressor to regulate embryonic development. It also functions as a transcriptional activator to regulate the expression of pluripotency genes. However, the molecular mechanism underlying the activation function of PCGF6 remains to be further understood.       In this study, we aim to explore the molecular mechanism of PCGF6 regulating pluripotency of mouse embryonic stem cells (mESCs). We knocked down Pcgf6 and found that it was not able to maintain the phenotype of mESCs, and the Oct4-GFP positive colonies were significantly reduced in the pre-iPSC reprogramming.        Further, PCGF6 was recruited by OCT4 to multiple super-enhancer (SE) regions upstream of proliferation genes, and promoted their expression levels and then enhanced the proliferation rate of mESCs. We analyzed the expression of SE-associated genes after Pcgf6 knockdown, and found that Pcgf6 knockdown downregulated those genes. By analyzing the promoter capture Hi-C data, we showed that PCGF6 activated those proliferation genes by regulating SE-promoter interactions in 3D chromatin.       Taken together, the role of PCGF6 in regulating pluripotency of mESCs is well established. We provide a potential insight into the new molecular mechanism of PcG components.主要报告内容:PCGF6经依赖超级增强子的染色质互作调控干细胞多能性        多梳蛋白家族环指蛋白PCGF6,是转录抑制复合物的核心组分之一,它传统的功能是作为转录抑制因子来调控胚胎发育。近期也有研究表明它可以作为转录激活因子来激活多能性基因的表达。然而,对于PCGF6的激活功能的潜在分子机制仍知之甚少。       本研究中,我们探索了PCGF6调控小鼠胚胎干细胞的多能性的分子机制。我们发现敲低Pcgf6后, mESCs的多能性表型不能维持, pre-iPSC重编程中的Oct4-GFP阳性克隆也显著减少。       更进一步地,我们发现PCGF6能被OCT4招募到许多增殖相关基因上游的超级增强子区域,促进这些基因的表达,进而提高mESCs的增殖速率。Pcgf6的基因敲低也使这些超级增强子相关基因下调。通过分析Promoter capture Hi-C数据,我们还发现这些基因的表达可以通过由PCGF6在三维水平上介导形成的超级增强子-启动子相互作用而被激活。       总之,PCGF6在mESCs的多能性调控中的作用显然十分重要,为未来研究PcG组分的调控机制方面提供了潜在的新思路。

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Opening of 2019 United Group Meeting 联合组会2019开幕

The Stem Cell United Group of Sun Yat-sen University Zhongshan School of Medicine was initiated and presided by professor Junjun Ding, taking the form of a lunch and once every two weeks.中山医学院干细胞联合组会由丁俊军教授发起和主持,每两周一次,以午餐会的形式进行。干细胞与再生医学平台的实验室人员轮流在联合组会上进行课题汇报,也会邀请校外嘉宾作汇报,目的为促进各个课题组之间的交流合作。The Stem Cell United Group for fall 2019 will be scheduled as follows:2019年的干细胞联合组会日程安排如下:On May 10th, professor Peng Xiang and professor Baomin Qin, respectively reported the subject and had an in-depth discussion with the audience.5月10日,项鹏教授和秦宝明教授分别汇报了课题,与听众展开了深入讨论。

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Sialation Review唾液酸化综述发表

Sialylation, or the covalent addition of sialic acid to the terminal end of glycoproteins, is a biologically important modification that is involved in embryonic development, neurodevelopment, reprogramming, oncogenesis and immune responses. In this review, we have given a comprehensiveoverview of the current literature on the involvement of sialylation in cell fate decision duringdevelopment, reprogramming and cancer progression. Sialylation is essential for early embryonic development and the deletion of UDP-GlcNAc 2-epimerase, a rate-limiting enzyme in sialic acid biosynthesis, is embryonically lethal. Furthermore, the sialyltransferase ST6GAL1 is required for somatic cell reprogramming, and its downregulation is associated with decreased reprogrammingefficiency. In addition, sialylation levels and patterns are altered during cancer progression, indicating the potential of sialylated molecules as cancer biomarkers. Taken together, the current evidences demonstrate that sialylation is involved in crucial cell fate decision.2018年11月26日本课题组关于唾液酸化修饰介导个体发育、体细胞重编程、癌症发生发展过程中细胞命运决定的综述在《Protein & Cell》上发表!唾液酸化修饰是一种糖基化修饰,唾液酸通过共价键连接到糖蛋白糖链的末端。唾液酸具有较强的负电性,在生理状态下电离出负电荷,并且位于糖链末端,因此,唾液酸化修饰可能在生物体生理功能发挥中起重要作用。目前已报道,唾液酸化修饰在早期胚胎发育、神经发育、体细胞重编程、癌症发生发展及免疫反应中起重要作用。本文主要阐述唾液酸化修饰在个体发育、体细胞重编程、癌症发生发展过程中细胞命运决定中的作用。唾液酸化合成酶UDP-GlcNAc 2-epimerase对小鼠早期胚胎发育至关重要,编码该酶的基因Gne敲除会引起小鼠胚胎在第10.5天死亡。唾液酸化糖基转移酶基因ST6GAL1敲低降低体细胞重编程效率。另外,唾液酸化水平和糖型在癌症发生发展过程中发生变化。因此,唾液酸化修饰与细胞命运决定息息相关。

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The Stem Cell United Group be Restarted干细胞联合组会再启动

The Stem Cell United Group of Sun Yat-sen University Zhongshan School of Medicine was initiated and presided by professor Junjun Ding, taking the form of a lunch and once every two weeks.中山医学院干细胞联合组会由丁俊军教授发起和主持,每两周一次,以午餐会的形式进行。干细胞与再生医学平台的实验室人员轮流在联合组会上进行课题汇报,也会邀请校外嘉宾作汇报,目的为促进各个课题组之间的交流合作。The Stem Cell United Group for fall 2018 will be scheduled as follows:2018年秋季学期的干细胞联合组会日程安排如下:On September 27, Yanan Zhang, the fifth year doctoral student of professor Peng Xiang's research group, and Chao Wei, the second year doctoral student of professor Junjun Ding's research group, respectively reported the subject and had an in-depth discussion with the audience.9月27日,项鹏教授课题组的博士五年级学生张亚南和丁俊军教授课题组的博士二年级学生魏超分别汇报了课题,与听众展开了深入讨论。

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Farewell Party of Brother Haopeng Yu于浩澎师兄欢送会

On June 14, 2018, the whole staff of the lab held a farewell party for the graduated brother Haopeng Yu!2018年6月14日,实验室全员为毕业的于浩澎师兄举行了欢送会!Brother Haopeng Yu made a farewell speech at the farewell party where food was very abundant.欢送会上食物非常丰富,浩澎师兄发表了离别感言。Speech by professor Ding丁老师讲话Everyone sang a song named "thousands of miles apart" for Brother Haopeng Yu.大家为浩澎师兄合唱一曲《千里之外》The group photo of the farewell party in the end! The unforgettable day!欢送会最后大家的合影!难忘的一天!

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Birthday Party of Professor Ding丁老师生日会

On March 26, 2018, all members of the research group held a warm birthday party for professor Ding in the laboratory!2018年3月26日,课题组全员在实验室为丁老师举办了一个温馨的生日会!We ordered a cake for Mr. Ding and sang a birthday song for him.大家一起给丁老师订了蛋糕,为丁老师唱了生日歌。Professor Ding was wishing丁老师许愿The writing on the cake was instructions of professor Ding to us蛋糕上写的字是丁老师对我们的教诲Professor Ding was going to eat the cake准备吃蛋糕的丁老师Happy selfies at the end of the birthday party生日会最后欢乐的自拍合影

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Professor Jianlong Wang was Invited to Report王建龙教授受邀汇报

On November 9, 2017, professor Junjun Ding invited professor Jianlong Wang, who is from the department of cell/major of development and regeneration at Icahn School of Medicine at Mount Sinai, the United States to presentation. The report on the topic of Transcriptional and Epigenetic Control of Stem Cell Pluripotency and Somatic Cell Reprogramming. Professor Jianlong Wang come from the department of cell/major of development and regeneration at Icahn School of Medicine at Mount Sinai, the United States, who graduated from Nankai University, graduated from Institute of Microbiology, Chinese Academy of Sciences with the master’s degree, and he received a doctor's degree at the university of Massachusetts at Amherst.Interaction between RNA viruses and host viruses to regulation of plant characterization is his main research. A post-doctoral fellow at harvard medical school's Boston children's hospital, focusing on the pluripotent regulatory network of embryonic stem cells, opened an independent laboratory at Icahn School of Medicine at Mount Sinai in 2009. His lab specializes in stem cell pluripotent regulation and molecular mechanisms in somatic reprogramming, focusing on transcription and epigenetic regulation. In five years, as the corresponding author, he published several articles in Nature, Nature Genetic, Cell Stem Cell, Molecular Cell and PNAS, and won the Harold and Golden Lamport award and the Irma t. Hirschl and weill-caulier Trusts Career Scientist award for his excellent work.2017年11月9日,丁俊军教授邀请美国西奈山伊坎医学院细胞/发育与再生医学系的王建龙教授到中山医学院作汇报,汇报题目为Transcriptional and Epigenetic Control of Stem Cell Pluripotency and Somatic Cell Reprogramming。王建龙是美国西奈山伊坎医学院细胞/发育与再生医学系的教授,他本科毕业于南开大学,硕士毕业于北京中科院微生物研究所,在马萨诸塞大学艾摩斯特分校获得博士学位,主要研究RNA病毒与宿主病毒的互作对植物表征的调控作用。在哈佛医学院波士顿儿童医院的从事博士后研究,重点关注胚胎干细胞的多能性调控网络,并于2009在西奈山的伊坎医学院开设独立实验室。他的实验室主要从事干细胞多能性调控和体细胞重编程过程中的分子机制研究,重点关注转录和表观遗传调控。5年内以通讯作者在Nature, Nature Genetic, Cell Stem Cell, Molecular Cell, PNAS发表多篇文章,同时获得了Harold and Golden Lamport奖,以及Irma T. Hirschl and Weill-Caulier Trusts Career Scientist奖,以表彰他的出色工作。Presided by professor Junjun Ding丁俊军教授主持Professor Jianlong Wang was mading an excellent presentation王建龙教授作精彩汇报The audience was actively asking and exchanging with professor Wang现场观众积极提问交流After the report, professor Jianlong Wang was invited to visit the laboratory汇报结束后邀请王建龙教授参观实验室

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Four-day Hong Kong Itinerary (Extreme Challenge)香港四日极限挑战

From 2017.08.07 to 08.10, all lab members went to Hong Kong for the first lab summer vacation. Excerpt the moments of lab members as a summary: Experience the beauty and slope of CUHKThe boss took a ton of selfies at the peak of TaipingDiscuss advertising and life in the Victoria Harbour at nightIt really only takes "20 minutes" to walk from Tsim Sha Tsui to MongkokHow many cans of wine we have to drink to pour out our tears?I still didn't dare to try the hottest chicken potThe LAN kwai fong dance floor at 2 am... That's really a big raise2017年8月7日至8月10日,实验室全员到香港进行了第一次实验室暑期出游活动!摘录同学的朋友圈作为总结:见识了港中大的美景和坡度老板大概在太平山顶拍了一吨自拍对着维港的夜景认真讨论广告和人生从尖沙咀走到旺角真的只用“二十分钟”要喝多少罐酒才能没有顾忌地倾诉和流泪呢结果还是没敢试最辣的那个鸡公煲凌晨两点的兰桂坊舞池…真让人涨姿势Going to Ocean Park when I grow up is like unlocking a new mapAlumnus Wei Chao resists badly under the park PendulumAlumnus Shaoshuai plays goats’ scream for the full jump on the JumperThe boss smiled and sat in the first row of the big roller coasterThunder beat... I knead my hip againAnother day ends with 25,000 steps长大后再去海洋公园就像解锁了新地图魏超师兄在大摆锤下抗拒得不行少帅师兄在跳楼机上全程鬼叫老板微笑着坐在了大过山车的第一排雷霆节拍…嘶待我再揉揉我的髋骨又是一天以两万五千步结束With tents and food, Hiking from the peak of the pyramid snake to pak tam auHands with feet, road with rubble,Beauty appears as expected in the dangerous peakWish a paraglider while standing in the windThe road down the mountain was so hard to walkMy fall almost scared alumnus to deathThe last section of cliff, boss grasps my hand tightlyThighs were shaking uncontrollably when landed on the beachCross the beach in the darkThe last hill road after the rain was wet and slipperyThe phone's light is just enough to light up a small patch in front of usFind a way to cross a log bridge when we are exhaustedArriving just in time to see the mountain side moonWhen we're lying on the beach, we're starlitThe fire rose with difficulty, and the roast was so sweetThe sea is cool at nightTogether with lab members, the beach was overwritten with struggleAnd the boss signed our wishSinging loudly in the moonlightThe seven tents joined together rattled in the windThe tents were beat crackling in the early morning rainLie back and remember the day... It feels too good to be true背着帐篷食材从北潭凹一路上了蚺蛇尖手脚并用,一路碎石,美景果然在险峰站在过山风中真希望自己有个滑翔伞下山的路实在难走得让人窒息摔了八九跤差点把师兄吓死最后一段峭壁老板一把抓住我的手落到在海滩上时大腿不可控制地在抖在夜色中横穿海滩下过雨的最后一个山头路很是湿滑手机的灯只够照亮眼前的一小块精疲力尽的时候发现还要过个独木桥到达的时候正好看见山边月出躺倒在沙滩上的时候,满目的星光月色火好不容易才升起来,烤肉真是香阿夜里的海水凉凉的跟师兄师姐一起在海滩上写满了努力奋斗老板还在我们的祈愿下签了个字月光下一首又一首地大声合唱连在一起的七个帐篷在海风里猎猎作响凌晨的大雨把帐篷打得噼里啪啦的躺着回想这样一天…觉得厉害得有些不真实Climb out of the tent after five to watch the sunriseThe distant sea surface grew warm under the glowSwam, Compared with Alumnus ShaoshuaiThe sea is too salty to be goodThe boss who couldn't swim forward was splashed a lot of waterThe picture of the alumni holding right is not suitable for young childrenIt's like having a lab meetingAn hour's ride back to Saigon is really excitingAlumnus Xingui walking down the street wearing nothing but swimming trunks is super coquettishThink about those four days slowly on my way backI feel really amazing about myself五点多就爬出帐篷想看日出霞光下远处的海面渐泛暖色跟少帅师兄比了一下游泳海水实在是咸的不行怎么努力也没法向前游的老板被泼了好多水师兄和师兄抱对的画面实在少儿不宜大家围在一起笑就像开组会一样坐一个小时的快艇回西贡真是赤鸡只穿泳裤走在街上的新贵师兄超级风骚Now think about it, I should certainly treasure the experienceThere's no need to be afraid and anxious all the timeBecause the very nice people are always here with me在回程的路上慢慢地想这四天觉得真把自己厉害坏了现在想想,给我的历练我当然应该珍惜也没必要一直害怕焦躁因为很好很好的大家一直都在我身边啊by 兔子(Rabbit)

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